In Progress

PCR primer search script

Program that searches PCR primers in a given dna sequence in Python

in the 5' - 3' strand and the reverse complement strand

No need for GUI

programs searches and gives the first hit as output for each strand

Primer is a piece of dna that has:

Length of 17 to 30 bases

GC-content about 50-60% (if possible)

The 3' end should have a G or C as the end base

Tm (melting temperature) between 55 and 65 ºC

Tm = 2ºC(A+T) + 4ºC (C+G) (A, T, C, G - number of respective bases)

Input:

Fasta file, or copy paste DNA sequence

output program:

Sequence primer 1 from 5' - 3':

Sequence primer 2 from 5' - 3':

Skills: Biology, Biotechnology, Python

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About the Employer:
( 1 review ) Utrecht, Netherlands

Project ID: #549938

Awarded to:

Redesha

I'm not a biology expert, so I do have a couple of extra questions and things to clarify if you pick me. I do however understand the jist of what you require, and am very experienced in Python. Hopefully we can get t More

$90 USD in 1 day
(3 Reviews)
2.8

6 freelancers are bidding on average $170 for this job

srinichal

I can do the project and waiting for more details

$220 USD in 2 days
(14 Reviews)
4.9
swwiz

I can make such a script and deliver by coming Tuesday morning.

$200 USD in 2 days
(1 Review)
4.7
lcruzc

I possess 4 years of experience in development in python, mysql and java. 1 year of experience in development in PHP and c++, and 2 years Django and postgreSQL, Web Designing. My strengths are in python programming, da More

$210 USD in 10 days
(0 Reviews)
0.0
iitmhimanshu

Hi, I am a graduate from Biotechnology department, IIT Madras. I have done primer search before also. Based on your detailed requirement, I can provide you exactly what you want. Kindly let me know your detailed More

$150 USD in 10 days
(0 Reviews)
0.0
hfeeki

I can do this project, Please see my pm, thanks.

$150 USD in 1 day
(0 Reviews)
0.0